KMID : 0043320100330081269
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Archives of Pharmacal Research 2010 Volume.33 No. 8 p.1269 ~ p.1275
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Neuroprotective effects of constituents of the root bark of Dictamnus dasycarpus in mouse hippocampal cells
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Jeong Gil-Saeng
Byun Erisa Li Bin Lee Dong-Sung An Ren-Bo Kim Youn-Chul
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Abstract
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Glutamate-induced oxidative injury causes neuronal degeneration related to many central nervous system diseases, such as Parkinson¡¯s disease, Alzheimer¡¯s disease, epilepsy and ischemia. The bioassay-guided fractionation of the EtOH extract of the root bark of Dictamnus dasycarpus Trucz. provided one neuroprotective limonoid, obacunone, together with a degraded limonoid, fraxinellone and two alkaloids, dictamine and haplopine. At concentrations of 100?150 ¥ìM, obacunone showed the potent neuroprotective effects on glutamateinduced neurotoxicity and induced the expression of heme oxygenase (HO)-1 in the mouse hippocampal HT22 cells. In addition, we found that obacunone increased p38 MAPK phosphorylation and induced HO-1 expression via p38 MAPK pathway. These results suggest that obacunone isolated from the root bark of D. dasycarpus increases cellular resistance to glutamate-induced oxidative injury in mouse hippocampal HT22 cells, presumably through the p38 MAPK pathway-dependent HO-1 expression. These results suggest that obacunone could be the effective candidates for the treatment of ROS-related neurological diseases.
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KEYWORD
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Glutamate-induced toxicity, Dictamnus dasycarpus, Obacunone, HT22, Heme oxygenase-1, p38 MAPK
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